This 12-page resource, from Frederick Community College, is a standard operating procedure (SOP) for viral gene delivery and evaluation by flow cytometry. The SOP outlines the purpose, scope, definitions, references, safety measures, responsibilities, materials, and equipment involved in the procedure. Step-by-step procedures are included for transduction of mammalian cells with the gene for green fluorescence protein (GFP) using adeno-associated virus (AAV) vector and subsequent evaluation of transduction efficiency using flow cytometry.

Procedure steps include: transduction preparation, transduction of HEK293 cells, cytometer setup and calibration, starting up the cytometer, preparing for instrument QC, running instrument QC, preparation of transduced samples, setting up the acquisition parameters on the cytometer, creating sample gating strategy on the cytometer, sample acquisition, data analysis, and exporting the data. Detailed steps are included.

This 15-page report, provided by the Cybersecurity Education for Advanced Manufacturing Organizations (CAMO) project, summarizes the build process for CAMO training scenarios, the results of implementing scenarios, and the lessons learned throughout. The training scenarios were developed by CAMO to address poor cybersecurity defenses in manufacturing organizations and the lack of security awareness among engineering technicians. The scenarios were designed to make cybersecurity instruction as accessible as possible, and can be used in both face-to-face and remote learning environments. The report provides an overview of the scenarios, discussing the content of each, their development, how to use them, and their impact on students. The scenarios have been used in high schools, two-year colleges, and four-year colleges. The report includes statistics on the efficacy of the each scenario. Also discussed in detail is the virtual industrial control system (ICS) designed for the project,...

This site, from Hitachi High Technologies America, focuses on the company's initiative to expand STEM education in classrooms. The site includes information about the project and Hitachi classroom visits, a calendar of STEM education events, and resources for educators, including papers and lesson plans for grades K-12 and beyond. Users are asked to complete a free registration process before accessing lesson plans.

This resource, published by Georgia Tech Research Institute, features an ecology and data visualization activity designed for seventh graders. In this activity, students color-code and quantify data from images of coral to evaluate the health of coral impacted by the Deepwater Horizon Oil Spill. The module covers basic ecology "concepts of ecosystems, abiotic and biotic factor, and benthic environments." Additionally, students learn to "define and quantify data as well as differentiate between temporal and spatial data." The activity meets the following learning requirement:

• Obtain, evaluate, and communicate information to examine the interdependence of organisms with one another and their environments.

The activity is divided into seven sections that last six class periods. The prep guide includes materials needed, planning, key questions and concepts, and grading opportunities for each section of the activity. The resource includes the following:

• An 28-page curriculum...

This resource includes a 199-page student laboratory manual and 244-page instructor guide. The lab manual provides assignments and labs corresponding to concepts found in the Introduction to Vacuum Technology E-book. Each activity unit includes a summary of learning activities, a pre-lab assignment, theoretical background needed for the activity, comprehensive instructions, and post-activity questions. The activities in the manual do not need to be paired with the textbook to be completed. The instructor guide mirrors the lab manual but includes "Instructor's Corner" sections that feature sample answers to the lab questions and tips for conducting the activities. The lab manual and instructor guide include the following activity categories, each with a certain number of individual activities:

• Learning Activity 1.1: Marshmallow Preparation - Measurements and Calculations,
• Learning Activities 2.1 - 2.4: Marshmallow Measurements, Pressure Calculation, Components of the Rough Vacuum...