A liquid bacterial culture is a bacterial culture grown in a liquid nutrient broth. There are many formulations for nutrient broth, but the most common is called Luria Broth or LB. LB is the type of broth we will be using in aseptic technique.

We use liquid cultures to easily transfer our growing bacteria as needed for our biotechnology experiments. Similarly to the streak plate we use an inoculating loop and a bunsen burner to transfer only the bacteria that we are working with.

Remember: The next step is to come into the lab to practice with the materials, view a demonstration, or to even just ask questions. Also, if you are ready to take the lab final, please inform your instructor. The lab final will be performed by yourself and will be graded with a rubric.

### Learning Objectives:

- 1.4 - Start liquid cultures.